What is Rhodamine 110, bis-(CBZ-L-alanyl-L-alanine amide)?

Rhodamine 110, bis-(CBZ-L-alanyl-L-alanine amide) is a rhodamine 110-based proteinase substrate that is a bisamide derivative of the fluorophore; peptides are covalently linked to each of the amino groups of rhodamine 110 (R110), thereby suppressing both the visible absorption and fluorescence of the dye. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is converted in a two-step process, first to the fluorescent monoamide and then to the even more fluorescent R110 (excitation/emission maxima ~499/421 nm. The substrate can be used to continuously measure the activity of elastase and closely related proteinases in cell extracts and purified enzyme preparations, using a fluorometer or fluorescence microplate reader.
This Molecular Probes® bisamide acquired of Rhodamine 110 is a acute and careful substrate for assaying proteases in band-aid or central active cells. These fluorogenic substrates accommodate an amino acerbic or peptide covalently affiliated to anniversary of R110’s amino groups. Upon enzymatic cleavage, the nonfluorescent bisamide substrate is adapted aboriginal to the beaming monoamide and again to R110, with a added access in fluorescence. The substrate can be acclimated to continuously admeasurement agitator action in corpuscle extracts and antiseptic agitator affairs application a fluorometer or fluorescence microplate reader, or can be acclimated for intracellular protease assays with assay by breeze cytometry or fluorescence microscopy.
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