About Pentagastrin

Pentagastrin is white or almost white powder. Can promote gastric acid and pepsin secretion of intrinsic factor, which is equivalent to gastrin secretion of endogenous gastrin 1/4, the role of sustainable 10 to 40 minutes. Mainly used in clinical gastric acid secretion function tests.

Properties:

The product is white or almost white powder; odorless.
The goods in dimethylformamide was dissolved in ethanol, slightly soluble, insoluble in water, soluble in dilute ammonia solution.
Specific rotation to take this product, accurately weighed, dissolved in dimethylformamide and quantitatively diluted into 1ml solution containing 10mg
Fluid, determined by law (Appendix Ⅵ E). Specific rotation of -25.0 ° to -29.0 °.

Identification:

(A) take the determination of the solution, according to spectrophotometry (Appendix Ⅳ A), at 230 ~ 350nm
Wavelength range absorbance was measured at 280nm and 288nm wavelength of maximum absorption at a wavelength of 275nm turn
Turning point.
(2) to take the product about 5mg, add hydrochloric acid solution (1 → 2) 0.5ml, mixing, and placing ampoules, sealed hydrolysis at 150 ℃
30 minutes, will be relocated hydrolyzate small beaker, evaporated on a water bath, dissolve the residue in water 0.1ml, as the test solution
Fluid. Take methionine, tryptophan, phenylalanine, aspartic acid and β-alanine, the amount of reference substance, add water were prepared
Containing 2.5mg per 1ml of the reference solution and the mixed solution containing 2.5mg of reference. Thin layer chromatography (Appendix Ⅴ B)
Determination, to learn the test solution, standard mixed solution and five kinds of reference solution 3μl, point in the same silica gel G
Plate with n-butanol - water - isobutyric acid - acetic acid (50:50:5:7) of the upper liquid as the agent, one-way three exhibition
Open, each made ​​about 15cm, after removing the developing solvent was evaporated to dryness and then as soon as the next expansion, and expand more than the previous number, the exhibition
Open, removed, dried, sprayed with ninhydrin solution (ninhydrin take 0.1g, add water and glacial acetic acid 0.2ml 0.4ml, plus three methylpyrazole
Piperidine 1ml, add ethanol into 20ml), heated at 70 ~ 80 ℃ for 15 minutes. Reference mixed solution should explicitly five spots, the test solution
Solution significantly the spot position and color should contact the respective main spots of the same reference solution.


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